Chlorambucilyl [3 H]phenylalanyl-tRNA crosslinking to 50 S ribosomal subunit proteins L4, L26-27 and L18-20
نویسندگان
چکیده
منابع مشابه
Crosslinking of Escherichia coli 50S ribosomal subunits with chlorambucilyl oligoprolyl phenylalanyl-tRNA molecular rulers.
A series of P-site probes, chlorambucilyl-(Pro)n-Phe-tRNAPhe, were prepared and reacted with poly(U)-directed Escherichia coli MRE 600 ribosomes. Upon binding of the probes to ribosomes, 90% of the cpm bound were not released following subsequent interaction with puromycin. In the absence of poly(U) or in the presence of poly(C), binding was limited to the amount of cpm bound if ribosomes were ...
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An affinity chromatographic procedure, using ribosomal or tRNA covalently coupled to agarose through an adipic acid dihydrazide spacer, has been developed to isolate and identify the Escherichia coli ribosomal proteins which specifically bind to these RNA molecules. Transfer RNA, 5-S, 16-S and 23-S ribosomal RNA were readily immobilized on agarose-dihydrazide: the maximum amount coupled decreas...
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Protein L27 has been implicated as a constituent of the peptidyl transferase center of the Escherichia coli 50 S ribosomal subunit by a variety of experimental observations. To define better the functional role of this protein, we constructed a strain in which the rpmA gene, which encodes L27, was replaced by a kanamycin resistance marker. The deletion mutant grows five to six times slower than...
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Binding of aminoacyl-tRNA with ribosomal subparticles seems to pass through two functionally important stages. At first the aminoacyl-tRNA binds with the 30 S-template complex by specific (codonanticodon) and non-specific (for different aminoacyltRNA) interactions with the 30 S subparticle itself in the so-called A-site. On further addition of the 50 S subparticle to the obtained ternary comple...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1981
ISSN: 0014-5793
DOI: 10.1016/0014-5793(81)80306-7